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Fig. 5.

ID
ZDB-IMAGE-200910-22
Source
Figures for Oppel et al., 2020
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Figure Caption

Fig. 5.

Evaluation and inhibition of the Ras signaling pathway in suz12-mutant and suz12-wild-type MPNSTs. (A) Immunohistochemistry analysis of signaling in three individual suz12-mutant (suz12+/−/−/−) and suz12-wild-type (suz12+/+/+/+) MPNSTs (n=3) each stained for phosphorylation of ERK, AKT, and S6 (p-ERK, p-AKT, and p-S6), indicating activation of Ras signaling. (B) The suz12a+/−, suz12b−/− (mut) or suz12-wild-type control MPNST (wt) tumor cell growth in the pericardial cavity of implanted embryos. These embryos were treated with DMSO vehicle control or PD-3025901 (PD901; 25 or 50 nM) (n=9 fish per treatment, doses based on the maximum tolerated dose of the individual drug). The fluorescent tumor area was determined for each embryo at 3 dpf (pre-treatment) and 7 dpf (post-treatment), and was reported as the normalized ratio of the red fluorescent area at 3  dpf versus 7 dpf in individual embryos. Individual values with medians (black bars) are shown. *P<0.05, **P=0.0064, ***P<0.0001 (Student's t-test). (C) Representative fish images at 3 dpf and 7 dpf after DMSO control or 50 nM PD0325901 treatment.

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