ZFIN Image: Wertman et al., 2020, Figure 5

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Figure Caption

Figure 5—figure supplement 2. Zebrafish pronephros histology does not look significantly different following treatment with cisplatin or either protective agent at 3 days post-treatment (dpt).

Casper zebrafish larvae were treated at 60 hours post-fertilization (hpf) with vehicle control or either 0.03 mM dopamine or 0.03 mM L-mimosine. Larvae were rinsed 12 hr later, then treated with either vehicle control or 0.125 mM cisplatin. Three days later, larvae were rinsed, sacrificed, then fixed overnight in 4% paraformaldehyde (PFA). The fixed larvae were then pre-mounted in 1.5% low-melting point agarose, then paraffin embedded, sectioned, and stained with H and E. Representative images are shown. (A) Untreated control, (B) Cisplatin alone, (C) 0.03 mM dopamine + cisplatin, (D) 0.03 mM L-mimosine + cisplatin. Yellow outlines highlight proximal tubular structures. Examination by a pathologist suggested that there were no obvious differences between treatment groups.

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