ZFIN ID: ZDB-IMAGE-200824-6
Figures for Johansson et al., 2020

Figure Caption/Comments:

Figure 6

prl3 Mutants Are Rescued by a V-ATPase Inhibitor

(A) Single embryo transcriptomics of heterozygote Tg(β-actin:prl3a) zebrafish reveals atpv1aa is selectively downregulated versus wild-type control siblings. Adjusted p-value (p < 0.05) was determined by DESeq2 using the Wald test with Benjamini-Hochberg correction for multiple testing. See also Table S5.

(B) g:Profiler output shows V-ATPase complex GO enrichment for Tg(β-actin:prl3a) differentially expressed genes versus wild-type controls. Inset: diagram of V-type ATPase, all genes encoding components labeled red are downregulated in Tg(β-actin:prl3a) expressing embryos.

(C) K-nearest neighbor graph (SPRING webtool) of prl3a expression in neural crest lineages using Wagner et al. (2018) zebrafish 24 hpf single-cell data.

(D) GSEA plotting enrichment of V-ATPase genes in the prl3a-negative subpopulation of pigment cells from Wagner et al. (2018).

(E and F) (E) Images and (F) quantification of melanocytes in wild-type (WT) or prl3a/ mutant embryo ± concanamycin A in a mitfavc7 regeneration assay, ANOVA using Tukey’s test, ∗∗∗∗p < 0.0001.

Figure Data:
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.