Figure 4

Figure 4

Germline base editing in pooled or individual embryos. Representative chromatograms of a wildtype embryo (A,D), pooled or individual F1 embryos at the twist2 (B,C) and ntl (E,F) loci are shown with the target window highlighted in yellow and target nucleotides boxed. Correct base editing for twist2 (C → T) and ntl (G → A) is observed as a trace peak in pooled embryos or as heterozygous peaks in individual embryos (ntl sgRNA is on the reverse strand and orientation shown is to depict the open reading frame). Incorrect bp changes and bystander edits are highlighted in red.