ZFIN Image: Hansen et al., 2020, Figure 5

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Figure Caption

Figure 5—figure supplement 1. Nanobody-based ciliary protein targeting in vivo.

(A) Nanobody localization in the neural tube of a zebrafish embryo. The mRNA of the anti-mCherry mNphp3(201)-VHH_LaM-2-eGFP (green) nanobody was injected into nacre zebrafish embryos. (B) Ciliary labeling (green) in the neural tube of b-actin:arl13b-gfp transgenic embryos (38). (C) RFP (red) expression in the neural tube of Ubi:zebrabow (40) transgenic embryos. (D) RFP (red) expression in the neural tube of Ubi:zebrabow (40) transgenic embryos, injected with mRNA of the anti-mCherry mNphp3(201)-VHH_LaM-2-eGFP nanobody. Scale bar: 20 μm, magnified view: 10 μm. Boxes indicate the position of the magnified views (bottom right panels). Arrow indicates the direction and the length of the shift of the respective fluorescence channel. The upper right panels show the individual RFP fluorescence, the bottom magnified view the overlay. A: anterior, P: posterior, L: left, R: right, D: dorsal, V: ventral. All images have been acquired from live embryos.

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