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Fig. 5 OFT endocardial proliferation depends upon functional inputs. (A-L) Lateral slices through the OFT at 51 hpf (as in Fig. 2A-C) illustrate EdU incorporation between 36 and 51 hpf in wild-type (WT) (A-C), tnnt2a mutant (D-F), myh7 mutant (G-I) and myh6 mutant (J-L) embryos. (M) Black and white bars in graph represent mean number of EdU+ and EdU− endocardial cells, respectively (mean±s.e.m.). EdU incorporation is significantly reduced in tnnt2a mutants (PI=10.2±4.3%) and myh7 mutants (PI=15.6±4.9%), but not in myh6 mutants (PI=27.8±6.3%), compared with WT (PI=29.6±3.7%) [n=17 (WT), 10 (tnnt2a), 13 (myh7), 7 (myh6); N=2; *P<0.05, **P<0.01; differences in PI compared with WT, Student's t-test]. Scale bar: 5 μm.