ZFIN Image: Choe et al., 2020, Figure 4.

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Figure Caption

Figure 4.

Spatio-temporal expression of olig2, rx1 and rx3, markers for diencephalon, retina, and optic primordia, in zebrafish embryos at 24 hpf. WISH was carried out with the following markers: olig2, rx1, and rx3 on each embryo (Figure 3 embryos were used). Overexpression of trim45 showed similar expression patterns with control for olig2 (A and B), rx1 (E and F), and rx3 (I and J). But, in knock-down, trim45 morphants showed compression of olig2 expression region and decrease in the size of thalamus (C). Also, knock-down of trim45 induced size reduction on eyes and decrease in transcript level of rx1 (G) and rx3 (K). Co-injection of trim45 mRNA with trim45-MO rescued the morphological phenotype of trim45 morphants (D, H and L). Lateral views – (A–D); anterior views – (E–L) th, thalamus; npo, neurosecretory preoptic area; pt, posterior tuberculum; ath, anterior hypothalamus. Scale bar – 200 μm (A–H), 100 μm (I–L).

Acknowledgments

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