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Figure 4 - figure supplement 3 Deficiencies in Fgf-dependent cell types in <italic>pgk1- / </italic>- mutants.

(A–D) Expression of neurod at 24 hpf showing lateral views of the olfactory epithelium (olf) (A, B) and dorsal views of the facial (fac) and glossopharyngeal (glos) ganglia (C, D) in wild-type and pgk1- / - embryos. Numbers indicate relative surface area of each expression domain (normalized to wild-type controls)± standard deviation and number of specimens. Expression domains are significantly smaller in pgk1- / - mutants (p<0.0001). (E, F) Dorsal view (anterior to the top) of anti-acetylated tubulin staining in the hindbrain region in a wild-type embryo (E) and pgk1- / - mutant (F). Positions of reticulospinal neurons (rsn) and trigeminal ganglia (tg) are indicated. Numbers indicate the mean number of neurons per side ± standard deviation and number of specimens. The number of neurons is significantly reduced in pgk1- / - mutants (p<0.0001).

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