Fig. 4

Fig. 4

(A) Dorsal view (3D reconstruction) of a pallial hemisphere imaged from a live casper;gfap:dTomato fish. The region analyzed is surrounded by the dotted line. The green and blue squared areas are enlarged in (B) and (D), respectively. (B) Example of an NSC that underwent two symmetric amplifying divisions. Green spots are superimposed to the NSCs of interest; tracks (green) with red dots indicating imaging sessions where the NSCs were still present (blue arrows point to the moments of image acquisition). (C) Relative proportions of the different types of NSC divisions inferred from their tracking in live fish (color coded). n, loss of gfap expression (NP/future neuron). n = 2 brains. (D) Example of a direct differentiation of an NSC (loss of gfap expression) (blue arrows). Asterisks (*) mark surrounding NSCs as visual landmarks. (E) Relative proportions of NSC fates leading to expansion (symmetric amplifying divisions; green), maintenance (asymmetric divisions; red), and consumption (symmetric neurogenic divisions and direct neuronal differentiations; blue). Light colors are the results of our simulations. Note the overall balance in NSC fates. n = 2 brains. Error bars, SD and credibility interval (see Supplementary Theory) for the in vivo data and the modeling, respectively. (F) Modeling of NSC dynamics. The inferred proportions and frequencies (in red, values per cell) of both rNSC and oNSC fates upon activation are indicated (oNSC ➔ oNSC + n, κ = 0.018 day⁻¹; oNSC ➔ n + n, μ₁ = 0.004 day⁻¹; oNSC ➔ n, μ₂ = 0.013 day⁻¹). The corresponding curves are plotted in green above experimental data in Fig. 3 (C, D, and F). Note that NSC/NSC fates monitored by live imaging include both rNSC/oNSC and oNSC/oNSC divisions.