Fig. 3

Fig. 3

(A) Dorsal view of a pallial hemisphere exemplifying some clones recovered at 64 dpi (magnification of the boxed area shows one clone). The analyzed region is outlined with dotted lines. (B) 3D reconstruction of clones at different time points illustrating the progressive increase in their NSC content until 183 dpi. (C) Time evolution of the number of NSCs (i.e., Sox2⁺ cells) per NSC-containing clone. (D) Time evolution of the proportion of NSC-containing clones among all clones. (E) Left: 3D reconstruction of a multicelled fully neuronal clone at 507 dpi. Right: Optical section along the plane defined by the dotted line on the 3D reconstruction (left). Note that the clone does not contain NSCs anymore and is completely detached from the ventricular zone (dotted line). (F) Time evolution of the ratio of neurons to NSCs within the entire population of traced cells. (G) Schematic illustrating the simplified model used for maximum likelihood estimation. The inferred activation frequency and relative proportions of both rNSCs and oNSCs are given in red and blue, respectively (rNSC ➔ rNSC + oNSC, ν = 0.007 day⁻¹; oNSC ➔ oNSC + oNSC, λ = 0.006 day⁻¹; oNSC ➔ loss, μ = 0.017 day⁻¹). (C and D) Box-and-whisker plots, experimental data. The central bold bar and the upper and lower edges of the boxes represent the means and SEM of the most likely clonal composition, respectively; the whiskers of the box respectively correspond to the 95% CIs of the most extreme clonal assignments still in agreement with clonality (see Supplementary Materials). They reflect the combined uncertainty stemming from the clonal reconstruction and the finite sample size. (C, D, and F) Clones as defined by the algorithm. n = 6, 3, 3, 3, 4, 6, 7, 9, and 7 brains at 6, 18, 30, 64, 91, 125, 183, 307, and 507 dpi, respectively. Solid lines depict modeling predictions.