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Figure 1—figure supplement 2 Morphogenesis of blastoderm explants prepared at 64 c, 128 c, 256 c or high stage.

(A) Bright-field single-plane images of bud stage blastoderm explants prepared from 64 c (n = 28, N = 3), 128 c (n = 34, N = 3), 256 c (n = 39, N = 3) or high (n = 17, N = 2) stage embryos. The white dashed lines outline the shape of the explant, while the white asterisks denote the main luminal cavity. Scale bar, 200 µm. (B) Percentage of extended or not-extended bud stage blastoderm explants prepared from 64 c (n = 28, N = 3), 128 c (n = 34, N = 3), 256 c (n = 39, N = 3) or high stage (n = 17, N = 2) embryos. (C) Circularity of bud stage blastoderm explants prepared from 64 c (n = 28, N = 3), 128 c (n = 34, N = 3), 256 c (n = 39, N = 3) or high stage (n = 17, N = 2) embryos. (D) Normalized extension length of extended blastoderm explants, prepared from 64 c (n = 20, N = 3), 128 c (n = 30, N = 3), 256 c (n = 31, N = 3) or high stage (n = 12, N = 2) embryos, at bud stage. (C,D) ns, not significant (ANOVA test).

Morphogenesis of blastoderm explants prepared at 64 c, 128 c, 256 c and high stage.

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