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Figure 3 (A) Bright-field single-plane images of wildtype (n = 120, N = 8), MZfz7a/b (n = 31, N = 3), MZwnt11 (n = 69, N = 5) and MZwnt5b (n = 75, N = 4) blastoderm explants at bud stage. Scale bars: 200 µm. (B) Percentage of extended or not-extended wildtype (n = 120, N = 8), MZfz7a/b (n = 31, N = 3), MZwnt11 (n = 69, N = 5) and MZwnt5b (n = 75, N = 4) blastoderm explants at bud stage. (C) Circularity of wildtype (n = 120, N = 8), MZfz7a/b (n = 31, N = 3), MZwnt11 (n = 69, N = 5) and MZwnt5b (n = 75, N = 4) blastoderm explants at bud stage. ****p<0.0001, *p=0.0424 (Kruskal-Wallis test). (D) Normalized extension length of extended wildtype (n = 108, N = 8), MZwnt11 (n = 46, N = 5) and MZwnt5b (n = 66, N = 4) blastoderm explants at bud stage. ****p<0.0001, *p=0.0461 (ANOVA test).