Figure 3

Figure 3

Effect of 5FU treatment on in vivo zebrafish model. (A) Tg(fli1:EGFP) zebrafish embryo (with green fluorescent vessels) xenotransplantated with Dil marked HT29 cells (red), injected into the duct of Cuvier (white asterisk). (B) The monitoring of zebrafish embryos showed viable cells after 24-48-72 h post-injection. (C) Analysis of Dil⁺ HT29 injected cells in zebrafish embryos, pre-treatment (time 0), after 24 h of treatment with DMSO (control group), IC₅₀ 2D, IC₅₀ 3D, and relative quantifications (scale bar = 100 µm). (D) Analysis of Dil⁺ HT29 injected cells in zebrafish embryos, pre-treatment (time 0), after 48 h of treatment with DMSO (control group), IC₅₀ 2D, IC₅₀ 3D, and relative quantifications (scale bar = 100 µm) (* p-value < 0.05; ** p-value < 0.01; *** p-value < 0.001).