ZFIN Image: Mashkaryan et al., 2020, FIGURE 9

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Figure Caption

FIGURE 9

Immunostaining for GFAP (gray) and GFP (green) coupled to TUNEL staining (red). (A) Wild type mouse hippocampus transduced with Lv-UbiC:GFP. (B) Higher magnification of the framed region in (A) without DAPI. (B′) Overlaid GFP and GFAP channels. (B′′) TUNEL staining as single fluorescence channel. (C) Wild type mouse hippocampus transduced with Lv-UbiC:IL4R-GFP. (D) Higher magnification of the framed region in (D) without DAPI. (D′) Overlaid GFP and GFAP channels. (D′′) TUNEL staining as single fluorescence channel. (E) Wild type mouse cortex transplanted with Lv-UbiC:GFP-transduced astroglia. (F) Higher magnification of the framed region in (E) without DAPI. (F′) Overlaid GFP and GFAP channels. (F′′) TUNEL staining as single fluorescence channel. (G) Wild type mouse cortex transplanted with Lv-UbiC:IL4R-GFP-transduced astroglia. (H) Higher magnification of the framed region in (G) without DAPI. (H′) Overlaid GFP and GFAP channels. (H′′) TUNEL staining as single fluorescence channel. Orange arrows show TUNEL-positive, transduced glia. All animals are WT. n ≥ 3 wild type animals. Scale bars: 100 μm.

Acknowledgments

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