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Figure Caption

Figure 5-figure supplement 1. Fluorescent immunohistochemistry in three-day old wild type and homozygous mutant <italic>slit3<sup>sa1569</sup></italic> labelled with tyrosine hydroxylase (<bold>A</bold>) and tubulin (<bold>B–F</bold>).

(A) Example of three Z-planes used to quantify catecholaminergic projections crossing the midline. (Stacks 1–3). (B) Anti-tubulin staining of slbpty77e/ty77e mutant larvae, known to have fewer neurons and axonal defects (Turner et al., 2019). (C–D) Anti-tubulin staining of wild type and homozygous mutant slit3sa1569. (E–F) Zoom-in of C-D for midline in the ventral forebrain, where slit3 is known to be expressed (Miyasaka et al., 2005).

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