Fig. 3

Fig. 3

a Heatmap depicting GSEA NES scores of mitochondrial pathways (Supplementary Data 2) in the ranked list of pairwise correlations between genes in the human liver, heart, or skeletal muscle (SKM) with either BR gene, known electron transport gene ATP5H or CDC42SE2, a representative non-mitochondrial candidate SEP. b SDS-PAGE of HEK293T treated with AMPK activator AICAR (500 µM) for the indicated time periods. c SDS-PAGE of HEK293T treated with indicated starvation regimes for the indicated time periods. Etomoxir was used at 3 µM. d Mitostress test (Agilent Seahorse) analysis of control versus stable BR knockdown U87MG cells. Oxygen consumption rate (OCR) reads were normalized with citric synthase (CS) activity. Data are mean and SEM of six technical replicates. Experiment is representative of two biological replicates. Oligo = oligomycin, FCCP = carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, Rot/AA = rotenone/antimycin A. e Analogous to d. except using short-term (72 h) siRNA-mediated depletion of BR. The experiment is representative of three biological replicates each with six technical replicates. Error bars represent SEM. f Spare respiratory capacity calculated from experiments in d and e. Data are mean and SEM of six technical replicates which are representative of two and three biological replicates for shRNA and siRNA-mediated depletion, respectively. p-values from two-sided unpaired t-test.