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Fig. 4-S2
High fat feeding did not induce EEC neogenesis.
( A–B) Epifluorescence image of in vivo EEC lineage tracing using Tg(neurod1:Gal4;cmlc2:EGFP); Tg(UAS:Kaede) (referred to as neurod1-Kaede). Before UV conversion, neurod1+ cells were labeled with green Kaede protein. Following UV exposure, green Kaede protein was converted into red Kaede protein and neurod1+ cells are labeled yellow. ( C) Confocal image of live neurod1-Kaede zebrafish intestine 0.5 hr post UV conversion. All the EECs are labeled. ( D) Confocal image of live neurod1-Kaede zebrafish intestine 2 days post UV conversion. Arrows indicate the EECs that were generated after UV conversion and exhibit green fluorescence only. ( E) Confocal image of live neurod1-Kaede zebrafish intestine 6 hr post high fat (HF) meal. No green EECs were detected. ( F) EEC neogenesis tracing at 0.5 hr, 6 hr and 2 days post UV conversion using the neurod1-Kaede system. ( G) EEC neogenesis tracing at 6 hr post HF feeding.