Figure 1

Figure 1

Grna and Grnb deficiency leads to transcriptional changes associated with an activated microglial state. (a) Schematic of a typical section from the adult zebrafish telencephalon. (b–c′) Micrographs depicting microglial cells in the telencephalon in Tg (mpeg1:mCherry) animals. (c,c′) Magnifications of areas boxed in (b) and (b′), respectively. Scale bars: 100 µm (b,b′) and 20 µm (c,c′). (d) Scheme depicting the isolation procedure of Mpeg1⁺ cells for transcriptome analysis. (e) Volcano plot of differentially expressed genes (DEGs) in mutant Mpeg1⁺ cells (padj ≤ 0.05; 1 ≤ log2FC ≤ −1). (f,g) Histograms depicting gene ontology (GO) terms enriched in the set of upregulated (f) and downregulated (g) genes in mutant Mpeg1⁺ cells.