ZFIN Image: Katsouda et al., 2020, Figure 3

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Figure Caption

Figure 3

Generation of 3-mst mutant zebrafish using the clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 approach. (A) Schematic representation of 3-mst-CRISPR-target site. (B) Gel electrophoresis of T7E1 assay in a representative sampling of CRISPR-injected and non-injected controls. (C) Type and frequency of alleles, identified in F1 generation. (D) Sequencing chromatograms of wild type and the mutant allele (aa102), selected for further studies, showing the presence of a premature stop codon.

Acknowledgments

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