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Fig. 7 Loss of vangl2 causes greater blebbing at late gastrulation. (A) Live control and vangl2vu67/vu67 mutant embryo images (reproduced from Fig. 2A) at the ypc/tb stage. White boxes indicate approximate position of microscopic analysis. D, dorsal. PCP quantitation of lateral mesodermal cells (vangl2vu67/vu67, n=180 cells, 4 embryos; vangl2vu67/vu67+ca-ezrb, n=175 cells, 4 embryos; vangl2vu67/vu67+fn1a/1b mRNA, n=150 cells, 3 embryos). (B) MLA data from A depicted as individual data points (in degrees). Long black line indicates 20°. (C) Percentage of cells forming bleb protrusions during the 15 min time-lapse imaging (vangl2vu67/vu67, n=560 cells, 4 embryos; vangl2vu67/vu67+ca-ezrb, n=560 cells, 4 embryos; vangl2vu67/vu67+fn1a/1b mRNA, n=560 cells, 4 embryos). (D) Live embryo DIC images of lateral mesodermal cells oriented as shown in A and Fig. 2A with dorsal to the right and anterior to the top. Wild-type and vangl2 mutant images are reproduced from Fig. 1A. Selected cells are outlined to show elongation and alignment relative to the dorsal-ventral body axis. Scale bars: 5 µm. (E) Cell migration tracks toward the dorsal body axis. Origins (black arrows) were standardized for comparison. (F) Directness values for individual cells (vangl2vu67/vu67, n=50 cells, 3 embryos; vangl2vu67/vu67+ca-ezrb, n=80 cells, 3 embryos; vangl2vu67/vu67+fn1a/1b mRNA, n=75 cells, 3 embryos). Box plot (C) shows the interquartile dataset, the median and the data range. Scatter plots (B,F) show individual data points and average values. **P<0.01, ***P<0.001, ****P<0.0001; unpaired Student's t-test with Welch's correction except B (Watson non-parametric two-sample U2 test).