Figure 5

Figure 5

Rag2^KI/KI mice develop severe cachexia syndromes. (A) Representative photographs of 2 months old Rag2^KI/KI mice and wild-type Rag2^+/+ littermates. (B) Skin was stripped from the hind limbs of 60 days old mice for gross comparisons of muscle mass. (C) Total body weight of 2 months old Rag2^+/+ mice (n = 9) Rag2^KI/KI mice (n = 9). (D) Representative photographs of lungs and livers from 2–5 months old Rag2^KI/KI mice and wild-type Rag2^+/+ littermates. (E) Representative H&E staining of lung and liver sections at 2–5 months old Rag2^KI/KI mice and wild-type Rag2^+/+ littermates. (F) Kaplan–Meier curves of Rag2^+/+ mice, Rag2^KI/KI mice and Rag2^−/− mice (Rag2^+/+ mice, n = 14; Rag2^KI/KI mice, n = 14; Rag2^−/− mice, n = 9). Chi square log-rank test was used for statistical analysis. The results are representative of three independent experiments. The error bars indicate the SDs. The data are presented as the mean ± standard deviation. *P < 0.05, **P < 0.01 and ***P < 0.001 by Student’s t test.