Figure 1

Figure 1

The recombination efficiency of zRAG is lower than that of mRAG at 37 °C. NIH3T3 cells were co-transfected with pCJGFP (mouse) (A,C), pSJGFP (mouse) (B,D), pCJGFP (zebrafish) (E,G) and pSJGFP (zebrafish) (F,H) and the indicated RAG1 and RAG2 using Lipo6000 transfection reagent. The GFP level was measured by flow cytometry to assess the recombination efficiency. The percentages of GFP positive cells were shown (the mean ± SD was calculated from triplicate experiments). (I,J) PCR analyses of the indicated D_HJ_H family rearrangements in RAG1-deficient pro-B cells (I) or RAG2-deficient pro-B cells (J). Input control: CD14 (bottom). PCR amplification was performed with fivefold serial dilutions of genomic DNA. The results are typical of three experiments. Bands related to rearrangements of various J_H segments are indicated on the left. The error bars indicate the SDs. The data are presented as the mean ± standard deviation. *P < 0.05, **P < 0.01 and ***P < 0.001 by Student’s t test.