Figure 2—figure supplement 8.

Figure 2—figure supplement 8.

Representative images of cells exhibiting labeling of nascent proteins in neurites. Maximum intensity projections of 3–6 confocal planes (5.7–12 microns) are shown. Freely swimming larvae were incubated with ANL for 24 hr (same larvae as in Figures 2 and 3). Click chemistry was performed to label nascent proteins prior to imaging. Nascent proteins in neuronal processes were detected in different brain regions including optic tectum (A, note the staining in the neuropil in the top left corner), Mauthner cells (B, C), hindbrain (D) and forebrain (E). Gray arrows indicate neurites. Scale bars = 5 μm.