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Figure 5
Fisetin decreased ROS expression level in cardiomyocytes. The cell permeable, nonfluorescent dihydrorhodamine 123 probe enters the cell where it is oxidized by ROS to fluorescent rhodamine 123. Fluorescence intensity, proportional to ROS expression level, was measured by flow cytometry in each experimental group. Normoxia: control group; HS/R: cells subjected to HS/R, treated with DMSO as vehicle control; HS/R + F: cells subjected to HS/R, treated with 15 μM fisetin. Results are expressed as the mean of three independent experiments. One-way ANOVA. Post-hoc analysis by Tukey. **P ≤ 0.01.
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