ZFIN Image: Chen et al., 2020, Fig. 1

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Figure Caption

Fig. 1 Generation of <italic>rpl18</italic> mutation using CRISPR/Cas9 system.

a, b Illustration describing the target site of rpl18. c, d Genomic sequences and predicted protein sequences based on the DNA sequence in wild-type and rpl18^−/− mutants. e qRT-PCR of rpl18 transcript levels of wild-type siblings and rpl18^−/− mutants at 24 and 48 hpf. qRT-PCR experiments were performed biological repeats and technical repeats in triplicate (N = 3). ***P < 0.001. f WISH results for rpl18 in siblings and mutants at 24 and 48 hpf, WISH results were verified three times independently (N = 3). All scale bars represent 250 μm.

Acknowledgments

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