Figure 5

Figure 5

Temperature-dependent heart rate modulation. (a,b) Heart rate response to temperature gradients in medaka (100–102 hpf, n = 47–48 at each temperature in a) and zebrafish (31–33 hpf, n = 43–48 at each temperature in b) recorded at 13 fps. Significant differences between heart rates at each temperature were tested with one-way ANOVA and pairwise comparisons using Student’s t-test and are given with *P < 0.05, **P < 0.01 and ***P < 0.001 (significant differences are indicated only for 1 °C-increments; P-values for all pairs of combinations in a,b are given in Supplementary Table S1 and S2, respectively); data is presented as box plots (median±interquartile range) and overlaid scatter plots of heart rate values.