ZFIN Image: Idigo et al., 2020, Figure 3

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Figure Caption

Figure 3 Expression analysis of the zebrafish <italic>eef1a</italic> genes

(A) Expression of eef1a1l1 in different early embryonic stages detected by RT-PCR. The other eef1a genes were undetected at these stages (data not shown). (B) Expression of eef1a genes in 24, 48 and 72 hpf developmental stages using RT-PCR. NTC, no template control. (C) Expression of eef1a genes in different tissues of adult zebrafish detected by RT-PCR. (D) Expression levels of eef1a genes in brain, muscle and liver tissues, and (E) comparison of the relative levels of their transcripts in these tissues. Expression values were normalised to those of ATPsynth, NADH and 16S. Results are means ± SD, n=3. *P<0.05; **P<0.01; ***P<0.0001. For comparison, data were presented as the gene expression ratio of the target mRNA to the geometric mean of reference genes for each tissue. (F) Western blot showing Eef1a2 expression in brain, liver and muscle zebrafish tissues using eEF1A2-Abcam antibody (1:1000). Control is muscle tissue from mouse. (G) Validation of eEF1A2-Abcam antibody (ab82912) specificity using lysates isolated from individually transfected HEK293T cells with GFP-tagged Eef1a constructs. Abbreviation: Zf, zebrafish.

Figure Data

Expression / Labeling details

Acknowledgments

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