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Figure 1 mRNA levels of act-5 and act-3 in WT and mutant alleles. qPCR analysis of act-5 (A) and act-3 (B) mRNA levels in WT and act-5(ptc), act-5(Δ1), and act-5(Δ2) mutants. act-3 mRNA levels are upregulated when act-5 mutant mRNA levels are reduced (i.e., only in the act-5(ptc) allele). WT expression levels are set at 1. Data are mean ± S.E.M.; average dCt values are shown in Figure 1—source data 1. Two-tailed Student’s t-test was used to calculate P values
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