Figure 1—figure supplement 1.

Figure 1—figure supplement 1.

(A) Schematic of all known act-5 isoforms and alleles used in this study. Black boxes indicate the deletion and nonsense alleles used in this study; black arrows point to the position of the qPCR primers. The act-5(ok1397) isoform was only identified in the act-5(Δ2) allele. (B) Partial sequence of act-5 (WT, ptc and Δ1 alleles). The PTC in the act-5(ptc) allele is 264 nucleotides from the next exon-intron junction and 888 nucleotides from the stop codon. Red indicates the mutation; PTC is underlined; ‘nnn’ and ‘---’ indicate deleted nucleotides in the act-5(Δ1) allele. The dt2017 deletion leads to a PTC which is located in the last exon (153 bases from the stop codon) and is thus predicted not to trigger NMD (Kashima et al., 2010; Lindeboom et al., 2016). act-5(dt2019) = act-5(ptc); act-5(dt2017) = act-5(Δ1); act-5(ok1397) = act-5(Δ2).