ZFIN Image: Kobayashi et al., 2019, Figure 4

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Figure Caption

Figure 4

Transplantation assays of HSPC populations. (a) Experimental procedure of a competitive repopulation assay. One hundred BFP-labeled donor cells were co-transplanted with 50,000 of DsRed-labeled KMCs (competitors) into a sublethally irradiated recipient animal. At 16 wpt, donor chimerism was determined by the percentage of BFP⁺ cells within the total BFP⁺ and DsRed⁺ cells in each recipient. (b) Representative result of FCM analysis in a recipient transplanted with gata2a⁺runx1⁺ cells. (c) Percentage of donor chimerism in each recipient group (mean ± s.e.m).

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