ZFIN Image: Kobayashi et al., 2019, Figure 1

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Figure Caption

Figure 1

Isolation of HSPCs from the zebrafish kidney. FCM analysis of KMCs was performed in a gata2a:GFP; runx1:mCherry double-transgenic zebrafish. (a) A scatter profile of KMCs. The SSC^low non-granulocytic cell fraction is gated. (b) SSC^low cells are subdivided into three distinct hematopoietic populations, gata2a⁺runx1⁺ (orange gate), gata2a⁻runx1⁺ (red gate), and gata2a⁺runx1⁻ (green gate). (c) Contour plot of FSC vs. SSC in KMCs, gata2a⁺runx1⁺, gata2a⁻runx1⁺, and gata2a⁺runx1⁻ cells. Dotted lines separate the “low”, “mid”, and “high” intensity of FSC.

Acknowledgments

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