ZFIN Image: Mosaliganti et al., 2019, Figure 5

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Figure Caption

Figure 5—video 1. Abscence of both regular and catch-up growth when salt transporters inhibited.

2D+time confocal movie showing growth and regeneration inhibition from ouabain treatment. Labelling by (Tg(actb2:Hsa.H2B-tdTomato)^hm25; Tg(actb2:mem-citrine)^hm26) from 30 to 34 hpf. Each frame has a spatial-sampling of 0.4 µm along X-Y axis, 1.0 μm along the Z-axis, and with temporal-sampling of 5 min across frames. The movie shows the transverse (XZ) and saggital (XY) views. The embryo was soaked in 100μM ouabain to inhibit Na⁺-K⁺-ATPase. The right vesicle of the embryo was punctured prior to imaging. At the start of the imaging session, the right ear is significantly smaller that the left ear. Throughout the imaging session, regular growth in the left ear and regeneration of the right ear are completely inhibited.

Acknowledgments

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