ZFIN Image: Chhabria et al., 2019, Fig. 2.

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Figure Caption

Fig. 2.

Effect of mannitol/glucose treatment with/withoutsodium nitroprusside(SNP)onklf2a:GFP expression and number of mural cellson thetectal vessels. (A) Representative micrographs of tectal vessels in Tg(klf2a:GFP) exposed to 20 mM mannitol or glucose co-treated with or without SNP. Scale bars: 20 µm. (B) Quantification of klf2a:GFP intensity in the tectal vessels (n=26 larvae/group). (C) Representative micrographs of tectal vessels showing separate and merged channels (green: fli1:GFF:UAS:GCaMP6; red: sm222ab:nls-mCherry ^sh480) for 20 mM mannitol- or glucose-exposed larvae co-treated with or without SNP. Scale bars: 20 µm. Arrows indicate mural cell nuclei. (D) Quantification of the number of sm22ab:nls-mCherry^sh480 nuclei on the tectal vessels for 20 mM mannitol- or glucose-exposed larvae co-treated with or without SNP (n=28 larvae/group). Data are mean±s.e.m. *P<0.05, **P<0.01.

Acknowledgments

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