IMAGE

Figure 2

ID: ZDB-IMAGE-191230-470
Publication: Tuttle et al., 2019 - Retrograde Ret signaling controls sensory pioneer axon outgrowth
All Figures: Figures for Tuttle et al., 2019

Image

Figure Caption

Figure 2 Ret loss-of-function alters pioneer axon growth cone morphology and protrusion.

(A,B) Lateral view, live image of collective pioneer axon terminals of wild-type siblings (A) and ret^hu2846 mutants (B) labeled by neurod:EGFP transgene at 30 hpf. (A’,B’) Individual pioneer axons labeled by mosaic expression of neurod5kb:mCherry plasmid (Arrowheads = examples of counted filopodia). (C,D) Quantification of distal collective axon terminal volume (C) or individual pioneer growth cone volume (D). ret^hu2846 mutants have significantly reduced collective axonal and individual growth cone volume. (E,F) Quantification of number (E) and length (F, n = filopodia counted) of filopodia (≥1 μm) per individual pioneer axon. Note that ret^hu2846 mutants have significantly reduced number of filopodia but not length. Error bars represent S.E.M.,**=p < 0.01, ***=p < 0.001, N.S. = not significant.

10.7554/eLife.46092.007Figure 2—source data 1.Individual embryo scoring data for scoring of growth cone and filopodia metrics in <italic>ret</italic> mutants.

These data can be opened with Microsoft Excel or with open-source alternatives such as OpenOffice.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife