ZFIN Image: Zwi et al., 2019, Figure 1

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Figure Caption

Figure 1

Chemical screen for modulators of zebrafish regulatory T cell (zTreg) recruitment in the regenerating tail. (a) Zebrafish tails were amputated at 4 weeks post-fertilization (wpf) along the dotted cut line. (b) Quantification was performed on the region from the amputation site to the tip of the regenerated tail at 3 days post-injury (dpi). (c) foxp3a: RFP+ zTreg cells were visualized by epifluorescence. (d) Normalized numbers of zTreg cells for each compound in the NIH Clinical Collection. Green line, statistical threshold for selecting compounds for retesting. (e) Screen hits were retested at 5 μM and 10 μM. Data presented as mean ± SEM; n = 5 per treatment group, n = 25 for controls. *** p < 0.001. None of the other treatments significantly increased the number of zTreg cells.

Acknowledgments

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