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Figure 8—figure supplement 1. Notch signalling regulates the expression of all Gli family members in the spinal cord.

Wild-type embryos were treated with DMSO, cyclopamine, or LY-411575 from 20 to 30 hpf, and stained with gli2a, gli2b or gli3 probes. Brackets in lateral views and dotted lines in transverse views denote the extent of the spinal cord. Arrows indicate gli2a expression in somites. Note that in LY-411575 treated embryos, gli3 expression is absent in most of the ventral spinal cord but is maintained in the very dorsal region. n = 15 embryos per staining. Scale bars: 20 μm.

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