ZFIN Image: Marques et al., 2019, Figure 5

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Figure Caption

Figure 5

Transmission electron microscopy (TEM) of fresh (control), vitrified and frozen primary growth (PG) oocytes of zebrafish (Danio rerio). (A–C) Fresh PG oocytes with normal cell organelles and intact plasma and nuclear membranes. (B,C) Presence of large number of mitochondria in the cytoplasm. (D–F) Vitrified PG oocyte showing an intact plasma membrane, chromatin condensation (arrow), signs of a ruptured nuclear membrane and a lower number of mitochondria. (G,H) Frozen PG oocyte showing a ruptured plasma membrane and a loss of intracellular contents (arrow). (I) Cytoplasm of a frozen PG oocyte with swollen mitochondria (arrow). PG = primary growth, Mt = mitochondria.

Acknowledgments

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