IMAGE

Figure 6

ID
ZDB-IMAGE-191230-1579
Genes
Antibodies
Source
Figures for Johansson et al., 2019
Image
Figure Caption

Figure 6

Ubiquitous Expression of GFP-Tagged Dkk1 Reveals Polarized Distribution of Dkk1 Protein at Tissue and Subcellular Levels

(A) Live Tg(hsp70l:dkk1b-GFP) embryos expressing Dkk1 (green) at different epiboly (EB) stages. On the right panel, higher magnification single-plane confocal image, of the area highlighted by the dashed frame, shows exclusion of Dkk1GFP from the axis, despite abundant extracellular Dkk1GFP in the surrounding non-axial tissue. The extracellular non-bound pool of Dkk1GFP is not detectable after fixation. See also Figure S2. Scale bar, 20 μm.

(B) Subcellular localization of Dkk1GFP in 80% epiboly (EB) and tail bud (TB) stage fixed embryos. Single-plane high magnification confocal images are shown of the region of interest in dashed white frames. Localization of Dkk1GFP to actin-rich membrane junctions was visualized by coexpression with red fluorescent protein-tagged actin-binding utrophin (RFP-UTR, red). Arrow highlights Dkk1GFP and RFP-UTR colocalization at a cell-cell junction. n = 6 for each condition. Scale bar, 20 μm.

(C) Dkk1GFP colocalizes with plasma membrane-associated β-catenin (red)-positive puncta. Embryos were fixed shortly after heat shock. Arrowheads highlight β-catenin positive puncta (red), which colocalize with Dkk1GFP (arrows).

(C’) Quantification of colocalization (n = 10). Mean Pearson’s R value is 0.60 for Dkk1-β-catenin and 0.03 for the randomized control. Scale bar, 20 μm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 51(6), Johansson, M., Giger, F.A., Fielding, T., Houart, C., Dkk1 Controls Cell-Cell Interaction through Regulation of Non-nuclear β-Catenin Pools, 775-786.e3, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell