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Figure 5 Hippo pathway effector Yap1 is active and Yap1 is essential for valve formation in the OFT. (A) Confocal z-sections of the double transgenic line 4xGTIIC:d2GFP; kdrl:membrane-mCherry counterstained with the Elnb antibody and focused on the OFT. Scale bar: 20 µm. (B) Example of the valve phenotypes (normal, thick, delayed and no valve) and quantification of the phenotypes in the yap1+/+ controls embryos, yap1+/- and in yap1-/- mutant embryos. Chi-square test. n.s.: non significant, ***: p<10−3. Scale bar: 10 µm. Results obtained from two independent experiments.
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