Figure 3

Figure 3

JS (V194A) and JATD (A199P) Mutations in CEP120 Reduce the Thermostability of the C2B Domain

(A) Thermal denaturation of human CEP120 C2B domain, WT (green), V194A (blue), and A199P (red) monitored using changes in heat capacity (left panel, DSC) or fluorescence (right panel, thermal scanning fluorimetry).

(B) Temperature dependency of NMR data of the human CEP120 C2B domain, WT, V194A, and A199P mutant. ¹H,¹⁵N BEST-TROSY NMR spectra of ¹⁵N-labeled WT (green), V194A (blue), and A199P (red) human CEP120 C2B at 293K (20°C), 308K (35°C), and 310K (37°C).

See also Figures S2 and S3.