Figure 6

Figure 6

Electrophysiological assay to identify drugs that rescue the scn1lab mutant epilepsy phenotype. (a) Electrophysiology recording were obtained with an electrode placed in the forebrain of 5 dpf agar-immobilized scn1lab larvae that had previously showed suppressed seizure-like behaviour in the locomotion assay. (b) Bar graphs show the frequency of epileptiform events in a 10 min recording epoch for scn1lab larvae exposed to clemizole analogues 4 (n = 15), 6 (n = 12), 20 (n = 9), 6-APB (n = 6), norfenfluramine (NorFA) (n = 8), methylergonovine (MeERGO) (n = 7) or scn1lab mutants (n = 15). The graph represents mean ± SEM and individual data points are shown. Kruskal–Wallis one-way analysis of variance was used to test for significance. *P < 0.05; **P < 0.01; ***P < 0.001. (c) Representative field electrode recording epochs (10 min) are shown for clemizole analogues 4, 6, 20, methylergonovine (MeERGO) and 6-APB. These compounds showed significant changes in the frequency of events compared to untreated scn1lab mutant zebrafish (red).