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Fig. S4

ID
ZDB-IMAGE-191104-13
Source
Figures for Liu et al., 2019
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Figure Caption

Fig. S4

(A) Time-lapse confocal images showing DFC migration in wild-type and  cxcr4a um20 mutant embryos on a  Tg(sox17: GFP) background from 75%–90% epiboly stages. Scale bar, 50 μm. (B) Sox17 expression was examined by in situ hybridization in wild-type and  cxcr4a um20  mutants at the 75% epiboly stage. (C–D) Wild-type and  cxcr4a-deficient  Tg(sox17: GFP) embryos were harvested at the 10-somite stage for immunostaining. KV cells were labeled using an antibody against GFP. Expression of the basal–lateral marker E-cadherin (C) and the apical marker aPKC (D) were visualized using the indicated antibodies. Scale bar, 20 μm. aPKC, atypical protein kinase; DFC, dorsal forerunner cell; GFP, green fluorescent protein; KV, Kupffer’s vesicle;  sox, SRY-box transcription factor; Tg, transgene.


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