ZFIN Image: Prummel et al., 2019, Fig. S4

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Figure Caption

Fig. S4

Trimming of +2.0drl to the minimal 432 bp LPM +2.4drl enhancer region

(a) Schematic representation of the -6.35drl locus including the PCR-based trimming approach to map the smallest LPM specific regulatory enhancer region; repetitive elements highlighted in blue. Sequence depicts the +2.0drl region in intron 1 (Danio rerio strain Tuebingen chromosome 5, GRCz11 primary assembly, NC_007116.7:61649227-61650194): blue text depicts repetitive elements, colored boxes depict predicted transcription factor binding sites (JASPAR database for vertebrates, 80-85% threshold), yellow frame marks the beginning of drl exon 2, and red brackets outline the sequence for the +2.4drl core sequence. (b) Expression of +2.4drl:EGFP at 5 ss; arrow indicates axial mesoderm expression, indicating increased promiscuity after trimming from the initial +2.0drl element. Scale bar 400 μm (b) and 80 μm (c).

Acknowledgments

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