|
Fig. S9
In situ hybridization assays for specialized zebrafish families In situ hybridization for multiple amphioxus stages for 11 genes whose zebrafish orthologous family has undergone specialization of at least one member. Only the positive control (Mlc, bottom) showed clearly restricted expression in amphioxus. The experiment was performed once, using 10 embryos per probe, all of which showed the same pattern for each distinct probe. In situ hybridization data for zebrafish was obtained from Zfin, with the exception of Kif5 genes 133 and the Atl strongly specialized ohnolog atl1 134, which have been reported before and are not shown here