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Fig. S2 Scxa is the more conserved homologue of the mammalian Scx. A. Clustal alignment of the translation product of the zebrafish scxa and scxb genes with representatives of other major vertebrate groups (human, mouse, chicken, Xenopus laevis and Fugu rubripes). B. Sequence pair distances of representative the above proteins using the Clustal method with PAM 250 residue weight table. C. Synteny diagram based on Genomicus software and Ensembl GRCz11 showing similar position of scxa gene inside intron 3 of the Bop1 gene on the other strand, as described for mouse Scx (11), whereas scxb locus show changes and rearrangements compared with mouse and human genomes. D-E. In situ hybridisation for scxa (D) and scxb (E) for 3 dpf embryos from a scxa+/- incross. Cranial expression is shown in ventral view. scxa mRNA levels in scxa mutant (11/50 embryos from the incross) are reduced compared with heterozygoute (29/50) and +/+ embryos (10/50), genotypes confirmed (D). scxb mRNA levels and spatial pattern do not differ between genotypes, genotypes confirmed (E). scale bars-100μM.