|ZFIN ID: ZDB-IMAGE-190819-6|
Immunoreactive score of immunohistochemistry for β-catenin reveals that AURKA(V352I) is significantly lower than control and AURKA(WT) at 3, 5, and 7 months. (A) Representative images of immunohistochemistry (IHC) staining for β-catenin in control, AURKA(WT) and AURKA(V352I) at 3, 5, 7, 9, and 11 months; (B) Statistical analysis of β-catenin immunostaining IRS score at 3, 5, 7, 9, and 11 months; (C) Statistical analysis of nuclear β-catenin immunostaining IRS score at 3, 5, 7, 9, and 11 months. IRS score of control fish (Tg(fabp10a:EGFP-mCherry) abbreviated as mC, AURKA(WT) and AURKA(V352I) is shown in red (3 M), orange (5 M), green (7 M), purple (9 M), and blue (11 M). Statistical analysis of results was performed using a two-tailed Student’s t-test. The error bar means standard deviation. Asterisks (*) represent the level of significance. * p-value ≤ 0.05; ** p-value ≤ 0.01; *** p-value ≤ 0.001.
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