Fig. S1

Fig. S1

Expression Patterns of rap1aa, rap1ab, and rap1b in Zebrafish Embryos and Generation of rap1b Mutant Zebrafish Lines, Related to Figure 1. (A-C) Expression patterns of rap1aa (A), rap1ab (B) and rap1b (C) in the wild type embryos at 12 (upper), 14 (middle) and 24 (bottom) hpf. Upper and middle panels, dorsal views of flat-mounted embryos with anterior to the left; bottom panels, lateral views with anterior to the left. The boxed areas are enlarged in the insets. Arrowhead and arrows indicate notochord and posterior lateral plate mesoderm, respectively. Scale 3 bars: 200 μm. (D) Expression patterns of rap1b in the posterior regions of wild type embryos at 17, 20, 24 hpf. Transverse images with dorsal to the top. Scale bars: 20 μm. (E) Upper, partial sequence of exon3 of the rap1b gene, showing the TALEN binding sites (underlined) and the spacer DNA containing the BbsI restriction site. Lower, partial sequences of exon3 of the rap1b gene in wild type and rap1bncv124 and rap1bncv125 mutants. The rap1bncv124 mutant carries a 10-bp deletion, while rap1bncv125 mutant has a 11-bp deletion and a 9-bp insertion. (F) Amino acid sequences encoded by wild type and the mutated rap1b genes. Mutated alleles in rap1bncv124 and rap1bncv125 encode 28 and 69 mutated amino acids (underlined) from Ile36, followed by premature stop codons (asterisks), respectively. (G) Genotype of wild type allele (+/+) and heterozygous (+/‒) and homozygous (‒/‒) alleles of rap1bncv124 (left) and rap1bncv125 (right) mutants as confirmed by digestion of PCR products containing a BbsI site as indicated in (E).