ZFIN Image: Elworthy et al., 2019, Fig. 4

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Figure Caption

Fig. 4

Endothelial‐specific excision of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 rescue transgene. A: SPIM stack projections of lateral views of the head of 7‐dfp ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−; ubi:ift54; fli1:clover) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (itf54−/−; ubi:ift54; fli1:clover; kdrl:cre) fish showing endothelial Clover and faint mcherry expression. The position of the eye is labeled for orientation. Endothelial‐specific Cre‐mediated recombination of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 transgene is marked by mcherry expression. B: At 10 dpf, mcherry expression is more pronounced than at 7 dpf

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Expression / Labeling details

Acknowledgments

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