ZFIN Image: Elworthy et al., 2019, Fig. 5

Image

Figure Caption

Fig. 5

Endothelial‐specific ablation of cilia does not perturb trunk or cerebral vascular development by 10 dpf. A: SPIM stack projections showing endothelial Clover expression in lateral views of the head, anterior trunk, posterior trunk, and tails of 10‐dpf Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (wt) (n = 9) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−;ubi:ift54) (n = 9) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−;ubi:ift54;kdrl:cre) (n = 4) fish. B: SPIM stack projections showing endothelial EGFP expression in dorsal views of the head of 10‐dpf Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:EGFP)y1^/+ (wt) (n = 4) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+; Tg(fli1a:EGFP)y1^/+ (ift54−/−;ubi:ift54) (n = 3) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+; Tg(fli1a:EGFP)y1^/+ (ift54−/−;ubi:ift54;kdrl:cre) fish (n = 12). EGFP, enhanced green fluorescent protein; WT, wild‐type

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.