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Fig. S4
To test if any random donor (tdTomato) insertion is seen when the donor is co-injected with a known Crispr (targeting golden locus) that is not specific to the eBFP2 target site, zebrafish embryos were co-injected with the following material: 1) (A) WT: Tomato 2) (B) WT: Golden Crispr/Ca9 + Tomato 3) (C) WT: Golden Crispr/Ca9 + Tomato + Drugs Multi-channel analysis of bright-field and red-fluorescence showed no red fibers in any of the experimental groups. Scale bars: 750 µm, main image and 250 µm, inset.
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